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ltb 5  (MyBiosource Biotechnology)

 
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    MyBiosource Biotechnology ltb 5
    Determination of inflammatory mediators. C57BL/6 mice were coinjected with 10 6 B16F10 apoptotic cells plus a sub-tumorigenic dose of 10 3 B16F10 viable melanoma cells in a subcutaneous tissue. After the coinjection, the mice were treated by gavage during five consecutive days with fish oil rich in omega-3 or soybean oil rich in omega-6 or a mixture of these oils in a ratio of 1:1. After 21 days, the tumors were removed and a homogenate was obtained to evaluate the concentration of LTB 4 ( A ), <t>LTB</t> <t>5</t> ( B ), the ratio between LTB 4 and LTB 5 (LTB 4 /LTB 5 ) ( C ), prostaglandin E 2 (PGE 2 ) ( D ), prostaglandin E 3 (PGE 3 ) ( E ), and the ratio between PGE 2 and PGE 3 (PGE 2 /PGE 3 ) ( F ) in the tumor microenvironment. All experiments were carried out using groups of five 6–8 week old female mice in, at least, two independent experiments. The risk value α was set at 5% ( p < 0.05). ** p < 0.01, *** p < 0.001.
    Ltb 5, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A Mixture of Polyunsaturated Fatty Acids ω-3 and ω-6 Reduces Melanoma Growth by Inhibiting Inflammatory Mediators in the Murine Tumor Microenvironment"

    Article Title: A Mixture of Polyunsaturated Fatty Acids ω-3 and ω-6 Reduces Melanoma Growth by Inhibiting Inflammatory Mediators in the Murine Tumor Microenvironment

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms20153765

    Determination of inflammatory mediators. C57BL/6 mice were coinjected with 10 6 B16F10 apoptotic cells plus a sub-tumorigenic dose of 10 3 B16F10 viable melanoma cells in a subcutaneous tissue. After the coinjection, the mice were treated by gavage during five consecutive days with fish oil rich in omega-3 or soybean oil rich in omega-6 or a mixture of these oils in a ratio of 1:1. After 21 days, the tumors were removed and a homogenate was obtained to evaluate the concentration of LTB 4 ( A ), LTB 5 ( B ), the ratio between LTB 4 and LTB 5 (LTB 4 /LTB 5 ) ( C ), prostaglandin E 2 (PGE 2 ) ( D ), prostaglandin E 3 (PGE 3 ) ( E ), and the ratio between PGE 2 and PGE 3 (PGE 2 /PGE 3 ) ( F ) in the tumor microenvironment. All experiments were carried out using groups of five 6–8 week old female mice in, at least, two independent experiments. The risk value α was set at 5% ( p < 0.05). ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: Determination of inflammatory mediators. C57BL/6 mice were coinjected with 10 6 B16F10 apoptotic cells plus a sub-tumorigenic dose of 10 3 B16F10 viable melanoma cells in a subcutaneous tissue. After the coinjection, the mice were treated by gavage during five consecutive days with fish oil rich in omega-3 or soybean oil rich in omega-6 or a mixture of these oils in a ratio of 1:1. After 21 days, the tumors were removed and a homogenate was obtained to evaluate the concentration of LTB 4 ( A ), LTB 5 ( B ), the ratio between LTB 4 and LTB 5 (LTB 4 /LTB 5 ) ( C ), prostaglandin E 2 (PGE 2 ) ( D ), prostaglandin E 3 (PGE 3 ) ( E ), and the ratio between PGE 2 and PGE 3 (PGE 2 /PGE 3 ) ( F ) in the tumor microenvironment. All experiments were carried out using groups of five 6–8 week old female mice in, at least, two independent experiments. The risk value α was set at 5% ( p < 0.05). ** p < 0.01, *** p < 0.001.

    Techniques Used: Concentration Assay

    Representative illustration of the main findings of our group. In ( A ), the previous results published by Bachi et al. are presented. In ( B ), the results are shown. Arachidonic acid (AA); –cyclooxygenase (COX); CXC motif chemokine ligand 1 (CXCL1) -; interleukin 10 (IL-10); eicosapentaenoic acid (EPA); –lipoxygenase (LOX); leukotriene B 4 (LTB 4 ); leukotriene B 5 (LTB 5 ); –prostaglandin E 2 (PGE 2 ); prostaglandin E (PGE ).
    Figure Legend Snippet: Representative illustration of the main findings of our group. In ( A ), the previous results published by Bachi et al. are presented. In ( B ), the results are shown. Arachidonic acid (AA); –cyclooxygenase (COX); CXC motif chemokine ligand 1 (CXCL1) -; interleukin 10 (IL-10); eicosapentaenoic acid (EPA); –lipoxygenase (LOX); leukotriene B 4 (LTB 4 ); leukotriene B 5 (LTB 5 ); –prostaglandin E 2 (PGE 2 ); prostaglandin E (PGE ).

    Techniques Used:



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    Image Search Results


    Determination of inflammatory mediators. C57BL/6 mice were coinjected with 10 6 B16F10 apoptotic cells plus a sub-tumorigenic dose of 10 3 B16F10 viable melanoma cells in a subcutaneous tissue. After the coinjection, the mice were treated by gavage during five consecutive days with fish oil rich in omega-3 or soybean oil rich in omega-6 or a mixture of these oils in a ratio of 1:1. After 21 days, the tumors were removed and a homogenate was obtained to evaluate the concentration of LTB 4 ( A ), LTB 5 ( B ), the ratio between LTB 4 and LTB 5 (LTB 4 /LTB 5 ) ( C ), prostaglandin E 2 (PGE 2 ) ( D ), prostaglandin E 3 (PGE 3 ) ( E ), and the ratio between PGE 2 and PGE 3 (PGE 2 /PGE 3 ) ( F ) in the tumor microenvironment. All experiments were carried out using groups of five 6–8 week old female mice in, at least, two independent experiments. The risk value α was set at 5% ( p < 0.05). ** p < 0.01, *** p < 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: A Mixture of Polyunsaturated Fatty Acids ω-3 and ω-6 Reduces Melanoma Growth by Inhibiting Inflammatory Mediators in the Murine Tumor Microenvironment

    doi: 10.3390/ijms20153765

    Figure Lengend Snippet: Determination of inflammatory mediators. C57BL/6 mice were coinjected with 10 6 B16F10 apoptotic cells plus a sub-tumorigenic dose of 10 3 B16F10 viable melanoma cells in a subcutaneous tissue. After the coinjection, the mice were treated by gavage during five consecutive days with fish oil rich in omega-3 or soybean oil rich in omega-6 or a mixture of these oils in a ratio of 1:1. After 21 days, the tumors were removed and a homogenate was obtained to evaluate the concentration of LTB 4 ( A ), LTB 5 ( B ), the ratio between LTB 4 and LTB 5 (LTB 4 /LTB 5 ) ( C ), prostaglandin E 2 (PGE 2 ) ( D ), prostaglandin E 3 (PGE 3 ) ( E ), and the ratio between PGE 2 and PGE 3 (PGE 2 /PGE 3 ) ( F ) in the tumor microenvironment. All experiments were carried out using groups of five 6–8 week old female mice in, at least, two independent experiments. The risk value α was set at 5% ( p < 0.05). ** p < 0.01, *** p < 0.001.

    Article Snippet: The concentration of the cytokines IL-1β, IL-6, IL-10, and CXCL1 (R&D System, Minneapolis, MN, USA) and the inflammatory mediators LTB 4 , LTB 5 , PGE 2 , and PGE 3 (MyBioSource, San Diego, CA, USA) were determined in the tumor homogenate by an ELISA kit.

    Techniques: Concentration Assay

    Representative illustration of the main findings of our group. In ( A ), the previous results published by Bachi et al. are presented. In ( B ), the results are shown. Arachidonic acid (AA); –cyclooxygenase (COX); CXC motif chemokine ligand 1 (CXCL1) -; interleukin 10 (IL-10); eicosapentaenoic acid (EPA); –lipoxygenase (LOX); leukotriene B 4 (LTB 4 ); leukotriene B 5 (LTB 5 ); –prostaglandin E 2 (PGE 2 ); prostaglandin E (PGE ).

    Journal: International Journal of Molecular Sciences

    Article Title: A Mixture of Polyunsaturated Fatty Acids ω-3 and ω-6 Reduces Melanoma Growth by Inhibiting Inflammatory Mediators in the Murine Tumor Microenvironment

    doi: 10.3390/ijms20153765

    Figure Lengend Snippet: Representative illustration of the main findings of our group. In ( A ), the previous results published by Bachi et al. are presented. In ( B ), the results are shown. Arachidonic acid (AA); –cyclooxygenase (COX); CXC motif chemokine ligand 1 (CXCL1) -; interleukin 10 (IL-10); eicosapentaenoic acid (EPA); –lipoxygenase (LOX); leukotriene B 4 (LTB 4 ); leukotriene B 5 (LTB 5 ); –prostaglandin E 2 (PGE 2 ); prostaglandin E (PGE ).

    Article Snippet: The concentration of the cytokines IL-1β, IL-6, IL-10, and CXCL1 (R&D System, Minneapolis, MN, USA) and the inflammatory mediators LTB 4 , LTB 5 , PGE 2 , and PGE 3 (MyBioSource, San Diego, CA, USA) were determined in the tumor homogenate by an ELISA kit.

    Techniques:

    Plasma concentrations of the leukotrienes ( a ) LTB-4 and ( b ) LTB-5 after treatment with palm oil TRF, carotenes, and dexamethasone (Dexa). Data are presented as the means ± SD, n = 10 rats per group. a P < 0.05 versus the control group, b P < 0.05 versus the OVA challenged group, c P < 0.05 versus OVA + TRF, d P < 0.05 versus OVA+ Carotene, e I < 0.05) versus OVA + Dexa.

    Journal: International Journal of Molecular Sciences

    Article Title: Effects of Palm Oil Tocotrienol-Rich Fraction (TRF) and Carotenes in Ovalbumin (OVA)-Challenged Asthmatic Brown Norway Rats

    doi: 10.3390/ijms20071764

    Figure Lengend Snippet: Plasma concentrations of the leukotrienes ( a ) LTB-4 and ( b ) LTB-5 after treatment with palm oil TRF, carotenes, and dexamethasone (Dexa). Data are presented as the means ± SD, n = 10 rats per group. a P < 0.05 versus the control group, b P < 0.05 versus the OVA challenged group, c P < 0.05 versus OVA + TRF, d P < 0.05 versus OVA+ Carotene, e I < 0.05) versus OVA + Dexa.

    Article Snippet: Standard enzyme linked immunosorbent assay (ELISA) kits used for the determination of rat IL-β, IL-6, IL-4, IL-13, TNF-α (Novateinbio, Woburn, Massachusetts, USA), CRP (eBioscience, Affymetrix, Santa Clara, California, USA), IgE, LTB-4, and LTB-5 (MyBioSource, San Diego, California, USA) were utilized.

    Techniques: